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Pulsatile secretion of LH in the ram: a re-evaluation using a discrete deconvolution analysis
M P Laurentie1, R Garcia-Villar, P L Toutain
1INRA, Station de Pharmacologie-Toxicologie, Toulouse, France.
The Journal of Endocrinology
|April 1, 1992
Summary
Pulsatile luteinizing hormone (LH) secretion in rams was analyzed using deconvolution. This method revealed shorter LH pulse durations than previously estimated, with pulse amplitude linked to prior quiescence, not subsequent duration.
Area of Science:
- Reproductive Endocrinology
- Animal Physiology
Background:
- Luteinizing hormone (LH) plays a crucial role in reproductive processes.
- Understanding LH secretion pulsatility is key to comprehending reproductive regulation.
Purpose of the Study:
- To accurately characterize LH secretion pulsatility in rams.
- To determine the instantaneous secretion rate profile of ovine LH (oLH).
Main Methods:
- Blood samples collected every 6 minutes for 24 hours.
- Plasma LH concentrations analyzed using deconvolution techniques.
- Individual kinetic parameters of oLH determined via intravenous injection.
Main Results:
- Deconvolution revealed a significantly shorter effective LH pulse duration (20.41 min) compared to traditional measurements (61.00 min).
- The number of LH pulses per 24 hours was consistent before and after deconvolution.
- Calculated LH production rate was 2.26 µg/kg/24h, with approximately 50% released during pulses.
- LH pulse amplitude and release amount correlated with the preceding quiescence period duration.
Conclusions:
- Deconvolution provides a more accurate assessment of LH pulse dynamics in rams.
- LH secretion is a non-periodic event, influenced by preceding physiological states.
- The findings enhance our understanding of the neuroendocrine control of reproduction in male sheep.