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Purification of green fluorescent protein overexpressed by a mutant recombinant Escherichia coli

Sulakshana Jain1, Sunita Teotia, Munishwar N Gupta

  • 1Chemistry Department, Indian Institute of Technology, Delhi Hauz Khas, New Delhi 110016, India.

Protein Expression and Purification
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PubMed

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...