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An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
Published on: August 18, 2018
Isolation of high quality RNA from Streptomyces
W Van Dessel1, L Van Mellaert, N Geukens
1Laboratory of Bacteriology, Rega Institute, K.U.Leuven, 10 Minderbroedersstraat, B-3000 Leuven, Belgium.
Journal of Microbiological Methods
|June 5, 2004
Abstract:
Several molecular techniques require high quality RNA, completely free of DNA. Standard methods to isolate total RNA from Streptomyces spp. are based on the application of a 'Modified Kirby Mix' [Practical Streptomyces Genetics. The John Innes Foundation, Norwich, pp. 613]. Here we present an alternative procedure using Triton X-100 and EDTA for the isolation of total RNA from Streptomyces.
