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Updated: Aug 24, 2026

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
Published on: October 21, 2016
Functional and physical interactions of Faf1p, a Saccharomyces cerevisiae nucleolar protein
Iwona Karkusiewicz1, Bozenna Rempola, Robert Gromadka
1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Abstract:
We report the discovery and characterisation of a novel nucleolar protein of Saccharomyces cerevisiae. We identified this protein encoded by ORF YIL019w, designated in SGD base as Faf1p, in a two hybrid interaction screen using the known nucleolar protein Krr1 as bait. The presented data indicate that depletion of the Faf1 protein has an impact on the 40S ribosomal subunit biogenesis resulting from a decrease in the production of 18S rRNA. The primary defect is apparently due to inefficient processing of 35S rRNA at the A(0), A(1), and A(2) cleavage sites.
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