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Related Experiment Videos

Magnetically labeled insulin-secreting cells.

Jose Oca-Cossio1, Hui Mao, Nata Khokhlova

  • 1Division of Endocrinology, Department of Medicine, University of Florida, Gainesville, FL 32610, USA.

Biochemical and Biophysical Research Communications
|June 5, 2004
PubMed
Summary

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Magnetic iron oxide nanoparticles (MION) can label insulinoma cells for MR imaging. This study confirms MION labeling does not affect cell insulin secretion or viability, enabling effective cell tracking.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Iron oxide nanoparticles are used for magnetic cell labeling and in vivo MR imaging.
  • Application in insulin-secreting cells is unexplored, raising questions about effects on insulin secretion.
  • Need to assess nanoparticle internalization, metabolic activity, and cell viability in these specific cells.

Purpose of the Study:

  • To investigate the effectiveness and consequences of labeling mouse insulinoma betaTC3 and betaTC-tet cells with monocrystalline iron oxide nanoparticles (MION).
  • To determine if MION labeling impacts insulin secretion, metabolic activity, cell viability, and apoptosis.
  • To evaluate the MR imaging contrast achievable with MION-labeled insulinoma cells.

Main Methods:

  • Exposure of betaTC3 and betaTC-tet cells to a 0.02mg/ml MION solution for 24 hours.

Related Experiment Videos

  • Assessment of MION internalization via microscopy.
  • Evaluation of metabolic and secretory activities, cell viability, and apoptosis compared to sham-treated controls.
  • Acquisition of MR images to assess contrast between labeled and unlabeled cells.

    • Main Results:

    • MION were successfully internalized by both betaTC3 and betaTC-tet cells.
    • MION-labeled cells exhibited metabolic and secretory activities statistically indistinguishable from sham-treated cells.
    • Cell viability and apoptosis rates remained constant, indicating no adverse effects from MION exposure.
    • MR imaging demonstrated significant contrast between MION-labeled and sham-treated cells.

    Conclusions:

    • Labeling murine insulinoma cell lines with MION is effective for MR imaging.
    • MION labeling does not hinder insulin secretion, metabolic activity, or cell viability.
    • This technique offers a promising method for tracking insulin-secreting cells in vivo using MR imaging.