Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A high-throughput NMR-based ee-assay using chemical shift imaging.

Manfred T Reetz1, Patrick Tielmann, Andreas Eipper

  • 1Max-Planck-Institut fur Kohlenforschung, D-45470 Mulheim/Ruhr, Germany. reetz@mpi-muelheim.mpg.de

Chemical Communications (Cambridge, England)
|June 5, 2004
PubMed
Summary

The throughput of a nuclear magnetic resonance (NMR)-based assay was significantly improved by adapting it for chemical shift imaging. This enhancement allows for the daily measurement of enantiomeric purity in thousands of samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel Ion Suppression-Based MS Approach (IS-MS) for Straightforward, Robust, and Rapid Identification and/or Differentiation of Liquid Samples: Wine as a Case Study.

Analytical chemistry·2026
Same author

Miniaturized one-step extraction for the analysis of tetrahydrocannabinol ∆<sup>9</sup>- and ∆<sup>8</sup>- isomers, phytocannabinoids, and hexahydrocannabinol in whole blood using liquid chromatography tandem mass spectrometry.

Journal of chromatography. A·2025
Same author

A high-resolution data set of fatty acid-binding protein structures. I. Dynamics of FABP4 and ligand binding.

Acta crystallographica. Section D, Structural biology·2025
Same author

Editorial.

Chimia·2025
Same author

Evaluation of ATR-FTIR, HPLC-DAD, GC-MS, and GC-IR for the Analysis of 145 Street Drug Samples From Drug Checking Services.

Drug testing and analysis·2024
Same author

Detection of N-desethyl etonitazene in a drug checking sample: Chemical analysis and pharmacological characterization of a recent member of the 2-benzylbenzimidazole "nitazene" class.

Journal of pharmaceutical and biomedical analysis·2024

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

Background:

  • Nuclear magnetic resonance (NMR)-based assays are valuable tools for chemical analysis.
  • Previous NMR assay throughput limited its application for large-scale sample analysis.
  • Enantiomeric purity determination is crucial in pharmaceuticals and chemical synthesis.

Purpose of the Study:

  • To enhance the throughput of a previously described NMR-based assay.
  • To adapt the NMR assay for chemical shift imaging to improve efficiency.
  • To enable high-throughput measurement of enantiomeric purity.

Main Methods:

  • Adaptation of a previously described NMR-based assay.
  • Implementation of chemical shift imaging techniques.

Related Experiment Videos

  • Utilizing a 19-capillary system for parallel sample analysis.

    • Main Results:

    • Achieved a throughput increase of at least 4-fold compared to the previous method.
    • Demonstrated the capability to measure the enantiomeric purity of 5600 samples per day.
    • Successfully applied chemical shift imaging to a multi-capillary NMR system.

    Conclusions:

    • Chemical shift imaging significantly enhances NMR-based assay throughput.
    • The developed method enables rapid and high-volume enantiomeric purity analysis.
    • This advancement facilitates large-scale screening and quality control in chemical and pharmaceutical industries.