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[Determination and stability studies of urea in urea creams by high-performance liquid chromatography]
1Analytical Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan.
Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|February 1, 1992
Abstract:
A method was developed for determining urea in urea creams by high-performance liquid chromatography using strong acidic cation exchange gel. Elution was carried out with 0.05 M phosphate buffer (pH 3.4) at 40 degrees C, and detection was made with a UV-spectrometer at 200 nm. This method was used for studying and comparing the stability of urea creams produced by ourselves and by other manufactures. The assay showed that there were differences in the stability of the tested creams.