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Updated: Aug 24, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
[Accelerated hydrophobic chromatography of plant extracts: a promising tool for study of biologically active
A A Markarian1, T D Dargaeva, R N Aliautdin
1I.M. Sechenov Moscow Medical Academy, Moscow, Russia 119881, Moscow, M. Trubetskaya, 8. markaria@rol.ru
Abstract:
A simple and efficient technique for fast isolation and purification of a total polyphenol fraction from dry herbal plant extract ("Phytoprost" preparation) has been developed. The method is based on sequential cascade of 1.0-5.0 kDa ultrafiltration and the following subsequent column chromatography of resultant oligo/polymeric compounds on Phenyl-Sepharose 4B-CL. This novel procedure allows to obtain a highly purified (not more than 0.5-0.6% of aromatic and heterocyclic impurities) fraction of plant polyphenols in amounts required for pharmacological/biochemical screening (0.85 +/- 0.06 A280/g dry extract) within 8-9 hours.
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