Related Experiment Video
Updated: Aug 24, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Single-molecule spectroscopy for nucleic acid analysis: a new approach for disease detection and genomic analysis
Peter M Goodwin1, Rhiannon L Nolan, Hong Cai
1Los Alamos National Laboratory, Bioscience Division, New Mexico 87545, USA. pmg@lanl.gov
Abstract:
Recently developed single-molecule spectroscopy (SMS) permits the analysis of fluorescent mixtures one molecule at a time. SMS methods provide the means to make rapid measurements on small, complex samples without the need for separations and target amplification enabling a new class of ultrasensitive nucleic acid assays. Here we give a brief overview of the current state of the art of SMS nucleic acid analysis and discuss ongoing work in our laboratory on two-color single-molecule fluorescence detection of specific nucleic acid sequences. In the future, two-color SMS nucleic acid assays will be used for a variety of applications including: gene expression analysis, disease detection and genomics.
Related Concept Videos
Sanger Sequencing
Modern Molecular Taxonomy
DNA Microarrays
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Applications Of NMR In Biology
The...

