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Updated: Aug 12, 2026
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
Ultra-sensitive fluorescence reader for bioanalysis
Jan Hesse1, Max Sonnleitner, Gerhard J Schütz
1Biophysics Institute, Johannes-Kepler-University Linz, Altenbergerstr.69, A-4040 Linz, Austria.
Abstract:
Recent advances in the development of new microscopical techniques with single-molecule sensitivity have given access to essentially new types of information on biological systems. In this review, basic methodological concepts of ultra-sensitive microscopy are presented and characterized, with focus on their applicability for a bioanalytical instrument. Measurements on artificial lipid bilayers were used to evaluate the feasibility of this novel technology. First examples of single molecule microscopy on cell membranes revealed new basic insights into the lateral organization of the plasma membrane.
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