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Updated: Aug 24, 2026

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
Published on: June 7, 2014
Precise determination of the complex optical constant of mica
Robert Nitsche1, Torsten Fritz
1Institut für Angewandte Photophysik, Technische Universität Dresden, D-01062 Dresden, Germany. nitsche@iapp.de
Abstract:
We present a general method for extracting optical constants n and k plus film thickness t of weakly absorbing materials that exhibit film thicknesses in the micrometer range. This method utilizes the simultaneous fit of multiple transmission measurements of different film thicknesses and employs the constraint that the frequencies of the corresponding measured interference patterns in the nonabsorbing wavelength region have to be matched by means of n, its derivative n' at a certain value E0, and t. Applying this method in practice, we calculate, in two different, independent ways, the optical constants of muscovite mica at 330-800 nm for thicknesses that range from approximately 5 to approximately 160 microns.

