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Updated: Aug 24, 2026
Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures
Published on: July 25, 2022
[Effect of static magnetic field on development toxicity of rat embryonic midbrain neurons cells]
Li-rong Duan1, Quan-yi Wu, Fang-ping Liu
1Department of Hygiene, School of Medicine Jiangsu University, Zhenjiang 212001.
Objective:
To explore the effect of static magnetic field (SMF) on the differentiation and proliferation of rat embryonic midbrain neurons cells.
Methods:
The micromass culture of rat embryonic midbrain neurons cell was applied to study the effect of varieties of SMF (1.0, 10.0, 50.0, 100.0, 200.0 mT) and FACS.
Results:
SMF inhibited the differentiation of the cell without affecting cell proliferation. The concentration of 50% inhibition of cell differentiation (ICD(50)) was 25 mT. The concentration of 50% inhibition of cell differentiation (IVD(50)) was 45 mT.
Conclusions:
The inhibition of SMF on embryonic midbrain neurons cells may be associated with impact of protein syntheses, and lipid peroxidation.
