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[The cloning of human augmentor of liver regeneration-interacting proteins]
Ming-hua Tong1, Si-qiang Chen, Ru-hua Yao
1Center of Infectious Diseases, The 458th Hospital of PLA, Guangzhou 510602, China. minghuat@163.net
Aim:
To screen human augmentor of liver regeneration(hALR)-interacting proteins and to explore the mechanisms of hALR in liver regeneration.
Methods:
The open reading frame of hALR was used to construct the "bait" plasmid and the genes encoding hALR-interacting proteins were screened from the human liver cDNA library that was pretransformed into yeast Y187 by yeast two-hybrid system. Bioinformatic analysis of the sequences of the positive clones were performed.
Results:
The positive clones encoding metallothionein, albumin, selenoprotein P,Na/K-ATPase, and 2 unknown proteins were screened out.
Conclusion:
The successful cloning of the genes encoding proteins interacting with hALR may pave the way for studying the interaction between the above proteins and hALR, and the molecular mechanisms of biological effect of hALR.
