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Updated: Aug 24, 2026
HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis
Published on: June 24, 2019
[Construction of recombinant vector pEGFP-ANG and expression of angiogenin gene in HUVECs]
Hong-bin Zhang1, Jie Wang, Jie Wu
1Department of Medical Research, General Hospital of Guangzhou Military Command, Guangzhou 510010, China. zhangwater@163.net
Aim:
To express the recombinant angiogenin protein in mammalian cells.
Methods:
Human ANG full-length cDNA was obtained by chemical synthesis. The target gene ANG was inserted into eukaryotic expression vector pEGFP-C2. The recombinant plasmid was transfected into the human umbilical vein endothelial cells (HUVECs) via Lipofectin transfection. The expression of ANG gene in transfected HUVECs was detected by fluorescence microscopy and immunohistochemical staining.
Results:
DNA sequencing showed the sequence of the synthetic ANG was correct and amino acid sequence of ANG was right. The green fluorescence could be seen in transfected HUVECs under fluorescence microscope. Immunohistochemical staining detection showed that ANG expressed in transfected cells.
Conclusion:
Human ANG full-length cDNA has been obtained. The ANG protein was expressed in mammalian cells successfully.
