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[Cloning and eukaryotic expression of mouse interleukin 21]
Guo-bing Chen1, Jun Dou, Jun-song Chen
1Department of Microbiology and Immunology, Medical College, Southeast University, Nanjing 210009, China. pawnking@vip.sina.com
Aim:
To clone mouse interleukin 21(mIL-21) gene and construct its eukaryotic expression vector.
Methods:
mIL-21 cDNA was amplified from ConA-activated mouse T cells by RT-PCR. And then the cDNA was cloned into eukaryotic expression plasmid pcDNA3.1 to construct recombinant plasmid pcDNA3.1/mIL-21 which was introduced into Sp2/0 cells by lipofectin. Expression of mIL-21 gene was detected by RT-PCR and NK cytotoxicity assay.
Results:
Recombinant plasmid pcDNA3.1/mIL-21 was constructed correctly and mIL-21 gene was expressed in transfected Sp2/0 cells.
Conclusion:
mIL-21 gene was cloned and expressed successfully, which lays the foundation for further studying anti-tumor effect of mIL-21 gene therapy in animal models.
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