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Updated: Aug 24, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
[Preparation and characterization of monoclonal antibodies against recombinant human NDPK-A]
Xue-ling Li1, Yi-fei Wang, Sheng Xiong
1Biomedicine Research & Development Center of Jinan University, Guangzhou 510632, China.
Aim:
To prepare monoclonal antibodies (mAb) against recombinant human nucleoside diphosphate kinase-A(NDPK-A) and characterize their properties.
Methods:
BALB/c mice were immunized with rhNDPK-A, and mAb was prepared by hybridoma technique. Ig subclass and specificity was analysed by double immunodiffusion and western blot respectively. The titres of mAbs in ascitic fluid, relative affinity and epitopes recognized by mAbs were determined by indirect ELISA.
Results:
6 hybridoma cell lines secreting anti-rhNDPK-A mAbs were obtained. Their Ig subclass belonged to IgG1. The titers of 6 mAbs in ascitic fluid were 1x10(-4)-5x10(-6). Relative affinity of mAbs were 4.5x10(-9)-2.8x10(-10) mol/L. They recognized 3 different epitopes on rhNDPK-A molecule.
Conclusion:
6 mAbs against rhNDPK-A have been prepared successfully which provide useful reagent for clinical diagnosis and further research.
