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Updated: Aug 24, 2026

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
Published on: September 2, 2019
[Cloning of a DnaJ homolog chaperon PBP and its subcellular localization]
Ying Liu1, Mei-cai Zhu, Yin-jing Wang
1Center for Clinical Molecular Biology Research, Airforce General Hospital of Chinese PLA, Beijing 100036, China. yingr21hotmail.com
Aim:
To isolate and identify a human DnaJ homolog chaperon, PBP, from a human skeleton cDNA library, and to analyze its expression and distribution in transfected mammalian cells.
Methods:
(32)p-dCTP labeled probe hybridization was used to screen the human skeleton cDNA library and sequence of the positive clones were analyzed. Then PBP gene was transfected into COS-7 cells using lipofectamin. PBP expressed in the cells were detected by Western-blot and indirect immunofluorescence staining.
Results:
A full-length(1.5 kb) cDNA of peripherin-binding protein (PBP) was identified, which is identical with that of mrj. Full length PBP was mainly localized to cytoplasms of COS-7 cells in interphase, and to nuclei in mitosis.
Conclusion:
The results indicate that besides cooperating with DnaK (HSP70), PBP itself plays an important role as a member of DnaJ family. PBP may also be involved in the regulation of cell cycle.
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