Related Experiment Video
Updated: Aug 24, 2026

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
Published on: July 1, 2018
[Expression and biological activities of TRAIL fusion protein gene]
Aim:
To obtain and express the full-length of TRAIL gene in prokaryocytes.
Methods:
The full-length of TRAIL gene was amplified from peripheral blood cells(PBCs)by RT-PCR and then cloned into the expression vector pGEX-2T. The TRAIL-GST was expressed in E.coli.
Results:
Sequence analysis indicated that the sequence of TRAIL gene was identical with that in the GenBank. The TRAIL-GST was expressed in E.coli. Expressed product could inhibit the proliferation of KB and Hela cells and finally lead to their apoptosis.
Conclusion:
The TRAIL-GST with biological activity has been expressed in the prokaryotic system.
More Related Videos
Related Concept Videos
Reporter Genes
Commonly used reporter...
Tagging and Fusion Proteins
What is Gene Expression?
What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
TGF - β Signaling Pathway
Recombinant DNA

