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[Typing for HLA-DR DNA by polymerase chain reaction with sequence-specific primers]
1Department of Urology Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. wuguojun@fmmu.edu.cn
Aim:
To type the HLA-DR DNA for renal transplantation by PCR with sequence specific primers (PCR-SSP).
Methods:
According to nucleotide sequences of HLA-DR, 16 pairs of specific primers and a pair of positive control primers were designed and synthesized for PCR-SSP. Then HLA-DR sites of 52 donors and recipients for renal transplantation were typed by the PCR-SSP.
Results:
All the samples were successfully typed by PCR-SSP with the synthesized primers. The results were available within 3 hours after sampling and the accuracy and reproducibility were 100%.
Conclusion:
Genotyping for HLA-DR sites by PCR-SSP with primers reported herein was a simple and accurate technique suitable for clinical application.