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[Stimulating the expression of macrophage inflammatory protein-2gamma by lipopolysaccharide in-vitro]
1Department of Laborotory Diagnosis, Changhai Hospital, Secondary Military Medical University, Shanghai 200433, China. yjhsmmu@yahoo.com.cn
Aim:
To study the expression level of macrophage inflammatory protein-2gamma(MIP-2gamma)stimulated by lipopolysaccharide(LPS)in-vitro.
Methods:
Murine monocyte macrophage like cell line RAW264.7 and BALB/c mice primary kidney cell culture were stimulated with LPS. MIP-2gamma mRNA expression level in these cells was detected by real time fluorescence quantitative RT-PCR.
Results:
MIP-2gamma mRNA expression level of RAW264.7 cell line increased quickly and reached the peak (7 times the normal level) within 2 hours after LPS stimulation and dropped to the base level within 16 hours, while that of cultured promary kidney cells of BALB/c mice elevated about 50 times compared with base level 12 hours following LPS stimulation.
Conclusion:
LPS can significantly stimulate the expression of MIP-2gamma mRNA, suggesting that MIP-2gamma may play some role in inflammatory process.
