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Characterization of GSTM3 polymorphism by real-time polymerase chain reaction with LightCycler

Paola Mozzoni1, Giuseppe De Palma, Eleonora Scotti

  • 1ISPESL Research Center, University of Parma, Via Gramsci 14, 43100 Parma, Italy.

Analytical Biochemistry
|June 9, 2004
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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