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Updated: Aug 24, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Molecular typing and phylogenetic analysis of enteroinvasive Escherichia coli using the fliC gene sequence
Juliana M K Amhaz1, Agda Andrade, Silvia Y Bando
1Departamento de Anàlises Clínicas e Toxicológicas da FCF-USP, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo (USP), Av. Prof. Lineu Prestes 580, 05508-900 São Paulo, SP, Brazil.
Abstract:
Non-motile enteroinvasive Escherichia coli (EIEC) is serotyped based only on O antigen polymorphism, since H antigen epitopes, present on the flagellins, cannot be characterised in these bacteria. In this study, we demonstrate the presence of the flagellin-coding fliC gene in non-motile EIEC strains. Moreover, we were able to group the 11 most common non-motile EIEC serotypes into six different RFLP patterns of the fliC gene. Amplicons representing each RFLP pattern were sequenced. Sequencing data were used to construct a phylogenetic tree which showed two main clusters: one sharing similarity with Shigella dysenteriae and pathogenic E. coli, and the other being closer to non-pathogenic E. coli.
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