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Benomyl-induced aneuploidy in mouse oocytes
1Department of Obstetrics and Gynecology, Louisiana State University Medical Center, Shreveport 71130.
Mutagenesis
|July 1, 1992
Summary
Benomyl, a fungicide, causes numerical chromosome changes in mouse oocytes by inhibiting cell division. This study highlights the in vivo mouse oocyte assay
Area of Science:
- Toxicology
- Genetics
- Developmental Biology
Background:
- Benomyl is a plant fungicide known to inhibit tubulin polymerization.
- It is recognized as a carcinogen and teratogen.
- Previous studies show conflicting results regarding its genotoxicity, specifically mutations and structural chromosome aberrations.
Purpose of the Study:
- To further define the genotoxic effects of benomyl.
- To evaluate the in vivo mouse oocyte assay for detecting chemicals that induce numerical chromosome changes.
- To investigate benomyl's ability to induce numerical and structural chromosome aberrations in mouse oocytes.
Main Methods:
- Female ICR mice were administered benomyl via oral gavage.
- Oocytes were collected 17 hours post-administration.
- Chromosome analysis was performed to assess numerical and structural aberrations.
Main Results:
- Benomyl exposure significantly increased the proportion of hyperploid oocytes in a dose-dependent manner.
- The percentages of hyperploid oocytes observed were 3.9%, 16.6%, 35.4%, 27.9%, and 29.4% for increasing doses.
- No significant increase in structural chromosome aberrations was detected.
Conclusions:
- Benomyl specifically induces numerical chromosome changes (aneuploidy) in mouse oocytes.
- The in vivo mouse oocyte assay is effective in detecting chemicals that cause numerical chromosome aberrations.
- These findings contribute to understanding benomyl's reproductive toxicity and genotoxic mechanisms.