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Preservation methodology for Rosellinia species
G Martijn ten Hoopen1, Juan Luis Ortíz, Maria Elena Aguilar
1CABI-CATIE-DGIS, c/o Centro Agronómico Tropical de Investigación y Enseñanza, 7170 Turrialba, Costa Rica. mthoopen@catie.ac.cr
Mycological Research
|June 10, 2004
Summary
Developing effective fungal preservation techniques is crucial for plant pathology research. This study optimized storage for Rosellinia isolates, finding that tailored methods, including liquid nitrogen, are essential for each species’ long-term viability.
Area of Science:
- Mycology
- Plant Pathology
- Microbial Preservation
Background:
- Fungal collections are vital for research but often lack adequate preservation methods.
- Maintaining fungal viability and minimizing changes are critical for accurate plant pathology studies.
Purpose of the Study:
- To identify optimal preservation techniques for three Rosellinia fungal isolates used in plant pathogenic research.
- To assess the suitability of various carriers and cryopreservation methods for Rosellinia species.
Main Methods:
- Tested inert and nutritious carriers (solid and liquid) for fungal conservation.
- Optimized liquid nitrogen storage by evaluating cryoprotectants, cooling, and thawing rates.
- Assessed fungal survival and growth rates over time for different storage conditions.
Main Results:
- Rosellinia bunodes showed limited survival (6-9 months) with traditional methods.
- Rosellinia necatrix and R. pepo were successfully stored for ≥16 months in various carriers, with R. necatrix viable for 2 years in silica gel.
- Liquid nitrogen storage yielded 100% survival for R. necatrix and R. pepo, though cryoprotectant and thawing rates impacted growth. R. bunodes storage was more challenging.
Conclusions:
- Generalised storage procedures are not feasible for these Rosellinia species.
- Successful long-term fungal preservation requires developing specific protocols tailored to individual isolates.
- Optimized cryopreservation is effective for R. necatrix and R. pepo, but requires careful selection of cryoprotectants and thawing rates for R. bunodes.