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RegB/RegA, a highly conserved redox-responding global two-component regulatory system.

Sylvie Elsen1, Lee R Swem, Danielle L Swem

  • 1Laboratoire de Biochimie et de Biophysique des Systèmes Intégrés (UMR 5092 CNRS-CEA-UJF), Grenoble, France.

Microbiology and Molecular Biology Reviews : MMBR
|June 10, 2004
PubMed
Summary

The RegB/RegA system in Rhodobacter regulates diverse energy processes. Redox signals from respiration control this system, impacting gene transcription across many bacteria.

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RedB, a Member of the CRP/FNR Family, Functions as a Transcriptional Redox Brake.

Microbiology spectrum·2022

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • The Reg regulon in Rhodobacter species controls essential energy processes like photosynthesis and respiration.
  • The membrane-bound sensor kinase RegB detects redox signals, likely from the aerobic respiratory chain.

Purpose of the Study:

  • To investigate the redox signal perception and regulatory mechanism of the RegB/RegA two-component system.
  • To understand the role of RegB autophosphorylation and its regulation.

Main Methods:

  • Analysis of mutations in cytochrome c oxidase affecting RegB autophosphorylation.
  • Investigation of the role of a redox-active cysteine in RegB's cytosolic region.
  • Characterization of the activity of phosphorylated and unphosphorylated RegA.

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Main Results:

  • RegB autophosphorylation is constitutively activated by mutations in cytochrome c oxidase, indicating the aerobic respiratory chain as the signal source.
  • A redox-active cysteine in RegB is involved in regulating its autophosphorylation.
  • Both forms of RegA (phosphorylated and unphosphorylated) modulate gene expression within the Reg regulon.

Conclusions:

  • The RegB/RegA system integrates redox signals from the respiratory chain to control gene expression.
  • Conserved RegB/RegA homologs suggest a fundamental role in bacterial redox-regulated transcription across diverse species.