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Related Experiment Videos

Methods for analyzing checkpoint responses in Caenorhabditis elegans.

Anton Gartner1, Amy J MacQueen, Anne M Villeneuve

  • 1Department of Cell Biology, Max Planck Institute for Biochemistry, Martinsried, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|June 10, 2004
PubMed
Summary

The Caenorhabditis elegans germ line provides a model to study DNA damage responses. Genotoxic insults trigger cell cycle arrest in premeiotic cells and apoptosis in meiotic cells.

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Area of Science:

  • Cell Biology
  • Genetics
  • Developmental Biology

Background:

  • Cells activate DNA damage checkpoint pathways to repair DNA, arrest the cell cycle, or undergo apoptosis.
  • The Caenorhabditis elegans germ line is a genetically tractable model for studying DNA damage responses in a multicellular organism.

Purpose of the Study:

  • To review the C. elegans DNA damage-response field.
  • To detail methods for assaying DNA damage responses in C. elegans.

Main Methods:

  • Utilizing genotoxic agents to treat C. elegans.
  • Observing cell cycle progression and apoptosis in germ cells.
  • Employing genetics- and genomics-based approaches.

Main Results:

Related Experiment Videos

  • Premeiotic C. elegans germ cells transiently halt cell cycle progression upon genotoxic insult.
  • Meiotic prophase germ cells undergo apoptosis in response to genotoxic agents.
  • Accumulation of unrepaired meiotic recombination intermediates also induces apoptosis in pachytene cells.
  • DNA damage-induced cell death and cell cycle arrest depend on conserved apoptosis and DNA damage checkpoint proteins.

    • Conclusions:

    • The C. elegans germ line effectively models DNA damage responses, including cell cycle arrest and apoptosis.
    • Conserved DNA damage checkpoint proteins are crucial for both cell cycle arrest and apoptosis.
    • Ongoing research is uncovering novel components of these response pathways using genetics and genomics.