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Isolation of photosystem II reaction center complexes from plants
Michael Seibert1, Inmaculada Yruela, Rafael Picorel
1Basic Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
Abstract:
Methods to isolate and purify 6- and 5-chlorophyll (Chl) D1/D2/Cyt b559 photosystem (PS) II reaction center complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material is also described. Criteria to assess the purity of photosystem II (PSII) reaction center preparations are presented, and problems associated with each of the isolation procedures are discussed.
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