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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
[Characterization of Vibrio cholerae O1 cultures isolated from environmental objects on the territory of the Russian
G G Onishchenko1, Iu M Lomov, B L Mazrukho
1Research Institute for Plague Control, Rostov-on-Don, Russia.
Abstract:
The biological properties of 46 V. cholerae O1 eltor cultures isolated in 2002 from water environment on the territory of Russia are presented. All isolated vibrios proved to be typical in their cultural, morphological, biochemical and serological properties. The atypical character of some of them was mainly linked with their phage resistance. The appearance of vibrios, sensitive to bacteriophage ctx+ and containing gene tcp in the absence gene ctx, was noted. Multilocus VNTR typing made it possible to group the cultures under study in 34 genotypes. The presence of toxin coregulated pili was found to be directly related to locus VcB. The necessity of the systematic study of the pheno- and genotypes of the isolated cultures with the aim of epidemiological surveillance of this infection is emphasized.
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