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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Saccharomyces bulderi sp. nov., a yeast that ferments gluconolactone
W J Middelhoven1, C P Kurtzman, A Vaughan-Martini
1Laboratorium voor Microbiologie, Wageningen Agricultural University, P.O. Box 8033, 6700 EJ Wageningen, The Netherlands. wout.middelhoven@algemeen.micr.wau.nl
Abstract:
An unknown yeast species was isolated from maize silage and was determined to be novel on the basis of morphological and physiological characteristics, nucleotide sequence of domain D1/D2 of LSU rDNA and from its electrophoretic karyotype. The name for the proposed new species is Saccharomyces bulderi Middelhoven, Kurtzman et Vaughan-Martini (type strain CBS 8638, NRRL Y-27203, DBVPG 7127). S. bulderi is closely related to S. barnettii and S. exiguus from which it can be distinguished by having a double vitamin requirement of biotin and thiamine and by no or slow aerobic growth on raffinose, a sugar that on the contrary is fermented rapidly. Gluconolactone is rapidly fermented with ethanol, glycerol and carbon dioxide being the main products.
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