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Updated: Aug 24, 2026

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
Published on: August 1, 2016
Incorporation of nonnatural amino acids into proteins
Tamara L Hendrickson1, Valérie de Crécy-Lagard, Paul Schimmel
1Department of Chemistry, 1Johns Hopkins University, 3400 N. Charles Street, Baltimore, Maryland 21218, USA. tamara.hendrickson@jhu.edu
Abstract:
The genetic code is established by the aminoacylation of transfer RNA, reactions in which each amino acid is linked to its cognate tRNA that, in turn, harbors the nucleotide triplet (anticodon) specific to the amino acid. The accuracy of aminoacylation is essential for building and maintaining the universal tree of life. The ability to manipulate and expand the code holds promise for the development of new methods to create novel proteins and to understand the origins of life. Recent efforts to manipulate the genetic code have fulfilled much of this potential. These efforts have led to incorporation of nonnatural amino acids into proteins for a variety of applications and have demonstrated the plausibility of specific proposals for early evolution of the code.
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