Interaction between morphology and angiogenesis in human early colorectal cancers

Morphologically, early colorectal cancers are divided into two types: polypoid cancers and non-polypoid cancers. They vary in growth pattern, progression, and genetic alteration. Angiogenesis between polypoid and non-polypoid cancers may also be different. Therefore, the present study aims to evaluate angiogenesis in the early stages of colorectal malignancy, with particular attention to the morphological differences. The serial slides of all materials (48 polypoid cancers, 10 non-polypoid cancers, 20 adenomas and 10 normal tissues) were immunohistochemically stained for three endothelial cell markers (CD31, von Willebrand factor and CD105), counted for the number of microvessels in the same hot spots, and the angiogenic status was estimated. Polypoid cancers had higher microvessel counts and were more predominantly supplied by activated (CD105-positive, newly forming) microvessels than non-polypoid cancers. The present study indicated the possibility that the difference in growth pattern might be explained by the difference in angiogenesis between polypoid and non-polypoid cancers.