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Related Experiment Videos

Serratia marcescens internalization and replication in human bladder epithelial cells.

Ralf Hertle1, Heinz Schwarz

  • 1Microbiology/Membranphysiology, University of Tübingen, Germany. ralf.hertle@mikrobio.uni-tuebingen.de

BMC Infectious Diseases
|June 11, 2004
PubMed
Summary

Serratia marcescens invades bladder cells using the ShlA toxin, which causes cell lysis and bacterial release. This pore-forming toxin is key to developing S. marcescens infections.

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Area of Science:

  • Microbiology
  • Cell Biology
  • Infectious Diseases

Background:

  • Serratia marcescens frequently causes catheterization-associated bacteriuria.
  • This bacterium adheres strongly to human bladder epithelial cells.
  • Bacterial internalization and lysis of epithelial cells are potential infection mechanisms.

Purpose of the Study:

  • To identify bacterial factors mediating S. marcescens internalization and epithelial cell lysis.
  • To investigate the role of hemolysin production in these processes.

Main Methods:

  • Gentamicin protection assay and electron microscopy were used.
  • Site-directed mutagenesis generated hemolysin-negative S. marcescens strains.
  • Internalization and cytotoxicity assays were performed on epithelial cell lines.

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Main Results:

  • The secreted cytolysin ShlA mediates S. marcescens internalization and epithelial cell lysis.
  • Microtubule and actin filaments are involved in bacterial invasion.
  • Hemolysin-negative mutants showed reduced cytotoxicity and internalization.
  • ShlA induces vacuolation, leading to epithelial cell lysis and bacterial release.
  • S. marcescens replicated within epithelial cells independently of hemolysin.

Conclusions:

  • ShlA is a pore-forming toxin that triggers microtubule-dependent invasion.
  • ShlA is the primary factor causing epithelial cell lysis, facilitating bacterial release.
  • ShlA plays a significant role in the pathogenesis of S. marcescens infections.