Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Leukocyte rolling on engineered nanodot surfaces.

Micro & nano letters·2024

Same author

Activation of Most Toll-Like Receptors in Whole Human Blood Attenuates Platelet Deposition on Collagen under Flow.

Journal of immunology research·2023

Same author

Anti-GPVI Fab reveals distinct roles for GPVI signaling in the first platelet layer and subsequent layers during microfluidic clotting on collagen with or without tissue factor.

Thrombosis research·2022

Same author

Thermal shift assay to probe melting of thrombin, fibrinogen, fibrin monomer, and fibrin: Gly-Pro-Arg-Pro induces a fibrin monomer-like state in fibrinogen.

Biochimica et biophysica acta. General subjects·2020

Same author

Opioid-prescribing patterns, storage, handling, and disposal in postoperative pediatric urology patients.

Journal of pediatric urology·2019

Same author

Platelet packing density is an independent regulator of the hemostatic response to injury.

Journal of thrombosis and haemostasis : JTH·2018

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Published on: June 23, 2017

Neutrophil string formation: hydrodynamic thresholding and cellular deformation during cell collisions.

K E Kadash¹, M B Lawrence, S L Diamond

¹Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

Biophysical Journal

|June 11, 2004

Summary

Neutrophils exhibit flow-enhanced adhesion, a phenomenon termed hydrodynamic thresholding, impacting their aggregation. This study reveals L-selectin

More Related Videos

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Published on: October 4, 2019

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Published on: November 4, 2022

Related Experiment Videos

Last Updated: Jul 10, 2026

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Published on: June 23, 2017

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Published on: October 4, 2019

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Published on: November 4, 2022

Area of Science:

Immunology
Biophysics
Cell Biology

Background:

Neutrophils display flow-enhanced adhesion, known as hydrodynamic thresholding, in various assays.
The efficiency of neutrophil collisions and adhesion under flow conditions is crucial for immune responses.

Purpose of the Study:

To investigate the mechanism of hydrodynamic thresholding in neutrophil-neutrophil interactions.
To determine the role of L-selectin and beta(2)-integrins in neutrophil adhesion and string formation.
To explore calcium mobilization as a mechanosensing response in neutrophils.

Main Methods:

Utilizing flow assays to measure primary collision efficiency and collision lifetimes.
Employing high-resolution imaging to observe neutrophil deformation during collisions.
Using antibodies to block specific cell adhesion molecules and assess their impact.
Analyzing secondary capture events and firm arrest on ICAM-1 and fibrinogen surfaces.
Monitoring calcium mobilization in neutrophils.

Main Results:

Primary collision efficiency showed a maximum at a wall shear rate of 100 s(-1), indicating hydrodynamic thresholding.
L-selectin antibodies significantly reduced collision efficiency, while antibodies against CD11a, CD11b, or CD18 had no effect.
Neutrophil-neutrophil string formation was observed on ICAM-1 but not on fibrinogen, peaking at 100 s(-1).
Neutrophils exhibited significant deformation during collisions at venous shear rates.
Neutrophils mobilized calcium upon adhesion, string formation, and subsequent flow onset, suggesting mechanosensing via beta(2)-integrin.

Conclusions:

Hydrodynamic thresholding influences neutrophil string formation, with L-selectin playing a key role.
ICAM-1 supports neutrophil string formation and firm arrest, unlike fibrinogen.
Neutrophil deformation and beta(2)-integrin-mediated mechanosensing are critical in adhesion dynamics.