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Updated: Aug 24, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis
Dihadenys Lemus1, Anandi Martin, Ernesto Montoro
1Instituto de Medicina Tropical 'Pedro Kouri', La Habana, Cuba.
Objective:
To study the performance of three rapid low cost methods for the detection of rifampicin resistance.
Methods:
A panel of 20 coded Mycobacterium tuberculosis strains was tested blindly by the low cost methods: nitrate reductase, MTT and resazurin assays, and compared with the results obtained with the gold standard methods: the proportion method on Löwenstein-Jensen medium and the BACTEC TB 460 system. We have also tested two commercial tests: MGIT and INNO LiPA Rif.TB kit.
Results:
Complete agreement was observed among all methods.
Conclusion:
These three simple methods might become inexpensive alternative procedures for rapid detection of rifampicin resistance in low-resource countries.
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