Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prospective, multicentre phase II study to evaluate the clinical benefit of reduced-dose lenalidomide and dexamethasone based on frailty stratification in elderly, unfit patients with newly diagnosed multiple myeloma.

British journal of haematology·2026
Same author

Phase II study of lenalidomide maintenance after rituximab-methotrexatebased induction therapy in primary central nervous system lymphoma.

Haematologica·2026
Same author

Treatment evolution and survival impact of consolidation therapies in mantle cell lymphoma: insights from an Asia-Pacific real-world registry.

Blood cancer journal·2026
Same author

Neutrophil-to-lymphocyte ratio half reduction as a surrogate of molecular response in polycythemia vera treated with ropeginterferon alfa-2b.

Therapeutic advances in hematology·2026
Same author

Brentuximab vedotin combined with cisplatin, cytarabine, and dexamethasone treatment in transplant-eligible Korean patients with relapsed or refractory Hodgkin's lymphoma.

Blood research·2025
Same author

Survival Benefits of Early Treatment With Blinatumomab in Adult Patients With Relapsed/Refractory B-Cell Acute Lymphoblastic Leukemia.

Clinical lymphoma, myeloma & leukemia·2025

Related Experiment Video

Updated: Jun 24, 2026

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
15:25

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Published on: March 16, 2010

Evaluation of AFLPs for germplasm fingerprinting and assessment of genetic diversity in cultivars of tomato

Young Hoon Park1, Marilyn A L West, Dina A St Clair

  • 1Department of Vegetable Crops, University of California, Davis 95616, USA.

Genome
|June 11, 2004
PubMed
Summary

Amplified fragment length polymorphism (AFLP) markers revealed limited genetic diversity in 74 tomato cultivars. AFLP fingerprinting successfully distinguished all cultivars, demonstrating its effectiveness for assessing genetic variation.

More Related Videos

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
06:41

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay

Published on: March 10, 2020

Related Experiment Videos

Last Updated: Jun 24, 2026

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
15:25

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Published on: March 16, 2010

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
06:41

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay

Published on: March 10, 2020

Area of Science:

  • Plant genetics
  • Molecular markers
  • Agricultural science

Background:

  • Cultivated tomato (L. esculentum L.) shows restricted genetic variation compared to wild relatives.
  • Understanding genetic diversity is crucial for crop improvement and breeding programs.

Purpose of the Study:

  • To evaluate genetic variation among 74 tomato cultivars using Amplified Fragment Length Polymorphism (AFLP) markers.
  • To determine if AFLP can generate unique fingerprints for individual tomato cultivars.

Main Methods:

  • Genotyping of 74 tomato cultivars using 26 AFLP primer combinations.
  • Analysis of 1092 scored bands, with 102 (9.3%) identified as polymorphic.
  • Calculation of pair-wise genetic similarity coefficients (Jaccard and Nei-Li).
  • Clustering analysis (UPGMA) and Principal Component Analysis (PCA) to group cultivars.

Main Results:

  • AFLP analysis identified 9.3% polymorphic bands across 74 cultivars.
  • Genetic similarity coefficients ranged from 0.16 to 0.98, with 72% of pairs exceeding 0.5.
  • Four main cultivar clusters were identified: I (vintage), II (modern hybrids), III (3 cultivars), and IV (2 cultivars).
  • Unique AFLP fingerprints were achieved for all 74 cultivars using a minimum of seven primer pairs.

Conclusions:

  • AFLP markers are effective tools for assessing genetic diversity in tomato germplasm.
  • AFLP fingerprinting provides unique identifiers for individual tomato cultivars, aiding in germplasm management.
  • The study highlights the utility of molecular markers in characterizing cultivated tomato genetic resources.