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Updated: Aug 17, 2026
Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Published on: February 8, 2016
[Expression of neuropeptide Y in rat testes and its significance]
Xiaowei Wang1, Yugui Cui, Ziqing Chen
1Key Laboratory, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Objective:
To assay the expression of neuropeptide Y (NPY) in rat testes and to investigate the significance of NPY in the regulation of androgen production and spermatogenesis.
Methods:
NPY mRNA levels in SD rat testes were measured by RT-PCR semi-quantity with beta-actin as internal control. NPY distribution was observed immunohistochemically.
Results:
NPY gene expressed in the testes, showing the strong positive band in the PCR production electrophoresis gel. In the immunostaining slides, NPY was found positively expressed in the Leyding cell area and around the testicular vessels and tubules, but not in the seminiferous tubules.
Conclusion:
There was positive expression of NPY in the rat testes, which showed that NPY played a direct role in the regulation of testicular function.
