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Published on: February 8, 2016
[Effects of c-jun on hCG-induced testosterone secretion of rat Leydig cells in vitro]
Shuanghu Yuan1, Sifan Xu, Xinhua Yang
1Department of Radiology, Shandong Tumor Hospital & Institute, Jinan, Shandong 250117, China. yuanshuanghu@sina.com
Objective:
To investigate the effects of c-jun on hCG-induced testosterone secretion in isolated rat Leydig cells by antisense oligodeoxynucleotides(ASODNs).
Methods:
c-jun ASODNs were used to antagonise the effects of c-jun, hCG was used to induce the testosterone secretion of LC cultured in vitro and testosterone was measured by radioimmunoassay.
Results:
The testosterone secretion of LC in vitro could be induced by hCG, which was a good model for the functional study of LC. c-jun ASODNs decreased the hCG-induced testosterone secretion of LC in a dose-dependent manner(P < 0.05).
Conclusion:
It is suggested that c-jun proto-oncogene enhances the testosterone secretion of LC.
Insights
The c-jun proto-oncogene enhances testosterone secretion in rat Leydig cells. Blocking c-jun with antisense oligodeoxynucleotides (ASODNs) reduced hCG-induced testosterone production, indicating c-jun
Area of Science:
- Endocrinology
- Molecular Biology
- Reproductive Science
Background:
- Leydig cells are crucial for testosterone synthesis.
- Human chorionic gonadotropin (hCG) is a key stimulator of testosterone secretion.
- The role of c-jun in regulating Leydig cell function requires further elucidation.
Purpose of the Study:
- To investigate the impact of c-jun on hCG-stimulated testosterone secretion in isolated rat Leydig cells.
- To determine if c-jun modulates Leydig cell responsiveness to hCG.
- To utilize antisense oligodeoxynucleotides (ASODNs) to specifically target c-jun expression.
Main Methods:
- Isolated rat Leydig cells were cultured in vitro.
- Testosterone secretion was induced using hCG.
- Antisense oligodeoxynucleotides (ASODNs) targeting c-jun were employed to inhibit its activity.
- Testosterone levels were quantified using radioimmunoassay.
Main Results:
- hCG effectively stimulated testosterone secretion in cultured Leydig cells, validating the experimental model.
- Treatment with c-jun ASODNs resulted in a dose-dependent decrease in hCG-induced testosterone secretion.
- A statistically significant reduction (P < 0.05) in testosterone was observed with c-jun ASODN treatment.
Conclusions:
- The c-jun proto-oncogene plays a significant role in enhancing testosterone secretion by Leydig cells.
- Inhibition of c-jun expression attenuates the stimulatory effect of hCG on testosterone production.
- These findings suggest c-jun is a key regulator in the signaling pathway of Leydig cell steroidogenesis.
