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[Activation of mouse macrophages by the alkali-extracted polysaccharide from spore of Ganoderma lucidum]
Qing-jiu Tang1, Jin-song Zhang, Ying-jie Pan
1Institute of Molecularbiology and Biochemistry, Free University Berlin, Berlin, Germany. tangqingjiu2003@yahoo.com.cn
Aim:
To investigate the activation of mouse macrophages by the alkali-extracted polysaccharides from the spore of Ganoderma lucidum (LZSBS).
Methods:
The mouse macrophages cultured in-vitro were stimulated by LZSBS. IL-1beta and TNF-alpha in the culture supernatants were detected by ELISA. NO production was detected by Griess assay. The percentage of phagocytosis of latex beads by mouse macrophages was counted under microscope.
Results:
The mouse macrophages stimulated by LZSBS increased in volume and darkened in appearance under phase-contrast microscope. LZSBS-activated mouse macrophages secreted IL-1beta and TNF-alpha produced a large amount of NO. The percentage of phagocytosis of latex beads by mouse macrophages was also significantly increased in the presence of LZSBS.
Conclusion:
LZSBS can activate markedly the mouse macrophages.
