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Published on: May 6, 2013
[Defect of TCR-mediated signal pathway of T cells in IDDM patients]
Hong-mei Li1, Ren-qian Zhong, Jia-ping Yu
1Department of Clinical Laboratory, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Aim:
To study the mechanism responsible for alteration of T cell response in IDDM patients.
Methods:
T cells from peripheral blood of IDDM patients were activated by anti-TCR antibodies. The level of TCR-mediated signaling pathway was analyzed.
Results:
T cells from IDDM patients responded weakly to anti-TCR antibody-induced proliferation, as compared with T cells from normal subjects (P < 0.05). The defect could be partially remedied by the addition of rIL-2, while the anti-CD28 antibody stimulation did not restore the proliferative response of anti-TCR-induced cells from IDDM patients (P = 0.03).
Conclusion:
Unresponsiveness of the T cells from IDDM patients to anti-TCR antibody may result from a defect in the signaling pathway, the CD28 co-stimulation-signaling pathway is normal. Defect in the TCR signaling pathway increases the sensitivity of T cells from IDDM patients to apoptosis or anergy.
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