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Updated: Aug 23, 2026
Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization
Published on: April 7, 2023
[Study on the mechanism of oocyte aneuploidy formation by multicolor fluorescence in situ hybridization]
1Department of Gynecology and Obstetrics, Tongji Hospital of Tongji Medical College, Huazhong Science and Technology University, Wuhan, Hubei, 430030 PR China. chenwenyyy@yahoo.com
Objective:
To investigate the mechanism of oocyte aneuploidy formation.
Methods:
The unfertilized oocytes were fixed 1-3 days after oocyte retrieval. Multicolor fluorescence in situ hybridization (M-FISH) was performed according to the Vysis protocol to check the chromosome status in oocytes by using centromeric enumerator probes for chromosomes 16, 18 and locus-specific probes for chromosomes 13, 21 and 22.
Results:
47% oocytes were found to be normal, while 53 % oocytes were abnormal. Nondisjunction was found in 22(18%) oocytes, unbalanced predivision of chromatids in 15(12%) oocytes, balanced predivision of chromatids in 45(36%) oocytes. The balanced predivision rate in oocytes aged in vitro>24h was much higher than that in oocytes aged in vitro
Conclusion:
Both nondisjunction, balanced and unbalanced predivision of chromatids are involved in the oocyte aneuploidy formation. Balanced predivision is related to the time in culture.
