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Updated: Aug 23, 2026
Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
[Polymorphism and multiplex amplification of 3 X-chromosome specific short tandem repeat loci]
Qiu-ling Liu1, De-jian Lü, Wei Cui
1Faculty of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, Guangdong, 510089 PR China. qiulinguser@tom.com
Objective:
To devise a multiplex PCR system of three X-chromosome specific short tandem repeat (X-STR) loci and study the genetic polymorphism.
Methods:
DXS6799, DXS6804 and DXS6854 were amplified simultaneously using a multiplex system and were typed by polyacrylamide gel electrophoresis and silver staining.
Results:
A total of 262 male and 255 female individuals from Guangdong Han population were tested; each locus showed 7 alleles. 73 haplotypes were detected in the male individuals. The haplotype diversity reached 0.9674.
Conclusion:
The 3 X-STR multiplex system is relatively abundant in polymorphic information for forensic identification and paternity testing.
