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Updated: Aug 23, 2026
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Published on: June 5, 2021
[The cloning, expression, purification and identification of SARS virus S2 gene and study on its immunological
Xue-yuan Dong1, Xiao-ang Yang, Yan Li
1Department of Immunology, Health Science Center, Peking University, Beijing 100083, China.
Aim:
To express S2 protein of SARS virus fused with Trx and then detect its reactivity to the sera from convalescent SARS patients.
Methods:
The Trx-S2 fusion protein was expressed in E.coli. After purification, the Trx-S2 fusion protein was detected by Western blot with 6 serum samples of convalescent SARS patients and 6 serum samples of healthy donors.
Results:
According to the SDS-PAGE analysis, the relative molecular mass (M(r)) of the Trx-S2 fusion protein is about 76 x 10(3). The fusion protein could react with all the sera from convalescent SARS patients but not with the sera from healthy donors.
Conclusion:
The Trx-S2 fusion protein provides a basis for the research on its role in the course of SARS virus infection of host cells and preparation of recombinant vaccine against SARS virus.
