[Acceleration of mixed lymphocyte reaction by HCV C-Fc gene-transferred murine dendritic cells]
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Published on: July 16, 2012
Quan-chu Wang1, Zhi-hua Feng, Yong-xing Zhou
1The Center of Diagnosis and Treatment of Infection Diseases of PLA, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. quanchuwang998@hotmail.com
Aim:
To observe the metergasis of murine dendritic cells (DCs) transfected with HCV C-Fc gene through electroporation.
Methods:
Mononucleocytes isolated from murine bone marrow were co-cultured with rmGM-CSF and rm-IL-4 for 7 days. Morphological characteristics of the cultured cells were observed under scan electron-microscope (SEM) and the expression of DEC205 on the cells was detected by FACS. DCs derived from the culture were transfected with plasmids containing HCV C-Fc gene. HCV C-Fc level in the transfected cells was detected by indirect immunofluorescence assay. MLR was studied with DCs and T cells.
Results:
Following 7-day culture, a large number of cells with typical characteristics of DC were observed. The HCV C-Fc level in the transfected DCs was higher. MLR was stimulated markedly by DCs transfected with HCV C-Fc gene in comparison with the control group.
Conclusion:
A large number of DCs could be generated from murine bone marrow mononucleocyte cultures supplemented with GM-CSF and IL-4 for 1 week. The function of DCs transfected with pcDNA3HCV C-Fc was enhanced in MLR.
