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[Preparation and the anti-tumor activity of mutant Staphylococcal enterotoxin B]
Xiao-hong Deng1, Yu-ling Zheng, Feng Yu
1College of Animal Science and Technology, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China. jiangyq@nic.bmi.ac.cn
Aim:
To prepare mutant Staphylococcal enterotoxin B(SEB) and observe its anti-tumor activity.
Methods:
The expressed mutant SEB-K172E in inclusion body was denatured and renatured, and then isolated and purified. The anti-tumor activity of the mutant SEB-K172E was compared with wild-type SEB.
Results:
Renaturation and purification method of the mutant SEB-K172E was developed. The anti-tumor activity of acquired mutant protein was ten times higher than that of wild-type SEB.
Conclusion:
The anti-tumor activity of the mutant SEB-K172E is much higher than that of wild-type SEB, suggesting that the mutant SEB-K172E may become a potential anti-tumor drug.
