Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RNA structure and function in C/D and H/ACA s(no)RNPs.

Anthony K Henras1, Christophe Dez, Yves Henry

  • 1Department of Chemistry & Biochemistry, UCLA Box 951569, 607 Charles E Young Drive East, Los Angeles, CA 90095-1569, USA.

Current Opinion in Structural Biology
|June 15, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A bifunctional H/ACA snoRNP mediates both pseudouridylation and rRNA scaffolding during ribosome assembly.

bioRxiv : the preprint server for biology·2026
Same author

The DExD-box RNA helicase Dbp7 unwinds short double-stranded RNAs.

Biochimie·2026
Same author

Ribosomal RNA synthesis by RNA polymerase I is subject to premature termination of transcription.

eLife·2026
Same author

Sni445 recruits box C/D snoRNPs snR4 and snR45 to guide ribosomal RNA acetylation by Kre33.

Nucleic acids research·2026
Same author

Ribosome biogenesis and homeostasis: in the front line to cope with cellular stress.

Biochimie·2025
Same author

Magnetoresistive detection of spin waves.

Science advances·2025

Small ribonucleoprotein particles (C/D and H/ACA types) are vital for RNA processing. Recent studies reveal sequence and structural elements critical for their function, localization, and assembly in various organisms.

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Biochemistry

Background:

  • C/D- and H/ACA-type small ribonucleoprotein particles (sRNPs) are essential for RNA processing across all domains of life.
  • These particles are involved in rRNA cleavage, chemical modification of various RNAs, and potentially mRNA processing.
  • Specific sequence and structural elements within the small RNAs of these particles dictate their function, localization, and assembly.

Purpose of the Study:

  • To highlight the importance of sequence and structural elements in C/D and H/ACA sRNPs.
  • To elucidate the role of novel sequence elements in sRNP localization and activity.
  • To provide evidence for the structural basis of C/D sRNP assembly.

Main Methods:

  • Analysis of sequence and structural elements in small RNAs from C/D and H/ACA sRNPs.

Related Experiment Videos

  • Identification of novel sequence motifs like the Cajal body box and m1/m2 boxes.
  • Structural analysis of conserved sequence motifs (C/D boxes) in C/D-type small RNAs.

    • Main Results:

    • A Cajal body box element mediates retention of specific H/ACA sRNPs within Cajal bodies.
    • The m1 and m2 boxes in snR30/U17 RNAs are crucial for early pre-rRNA cleavage and 18S rRNA maturation.
    • Conserved C and D motifs in C/D small RNAs form a kink-turn structure essential for particle assembly.

    Conclusions:

    • Sequence and structural features of small RNAs are critical determinants of C/D and H/ACA sRNP function and localization.
    • Novel elements like the Cajal body box and m1/m2 boxes reveal specific regulatory mechanisms.
    • The kink-turn structure provides a fundamental framework for C/D sRNP assembly.