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Cloning and nucleotide sequence of the 5' part of v-myb cDNA
P Bartůnĕk1, Z Kozmik, M Dvorák
1Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Virology
|October 1, 1992
Abstract:
cDNA of a subgenomic v-myb mRNA from AMV-transformed BM-2 cells was cloned. Sequencing of the 5' end of this cDNA revealed the structure of both AMV leader and the splice junction in v-myb message. The leader is a novel variant of known avian retrovirus leaders. The long open reading frame in the cloned cDNA starts with six gag-derived codons spliced to the myb-specific sequence.