Simple method for the assay of eperezolid in brain heart infusion broth by high-performance liquid chromatography
Jian Li1, Craig R Rayner, Roger L Nation
1Facility for Anti-infective Drug Development and Innovation, Victorian College of Pharmacy, Monash University, Parkville, Vic. 3052, Australia. jian.li@vcp.monash.edu.au
Abstract:
A sensitive high-performance liquid chromatography (HPLC) method was developed and validated for quantification of eperezolid in Brain Heart Infusion (BHI) broth. Linezolid was employed as internal standard and sample deproteinization with methanol was used. Calibration standards ranged from 0.10 to 20 mg/l. Recovery was approximately 100% at the concentrations examined. Eperezolid was stable in the autosampler vial for at least 72 h at ambient temperature and in BHI broth for 72 h at 37 degrees C. The intra- and inter-day accuracy and reproducibility (relative standard deviation, R.S.D.) were less than 12.3%. This assay is rapid and ideal for analysis of a large number of samples.
