Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

Production of Pharmaceuticals

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The yield of CT head scans for intracranial haemorrhage in anticoagulated patients presenting with head injury.

Clinical radiology·2026

Same author

Dual-Baseline Search for Active-to-Sterile Neutrino Oscillations in NOvA.

Physical review letters·2025

Same author

Search for CP-Violating Neutrino Nonstandard Interactions with the NOvA Experiment.

Physical review letters·2024

Same author

Inflammatory myofibroblastic tumours of the liver - a systematic review.

South African journal of surgery. Suid-Afrikaanse tydskrif vir chirurgie·2024

Same author

Measurement of the ν_{e}-Nucleus Charged-Current Double-Differential Cross Section at ⟨E_{ν}⟩=2.4 GeV Using NOvA.

Physical review letters·2023

Same author

Search for Active-Sterile Antineutrino Mixing Using Neutral-Current Interactions with the NOvA Experiment.

Physical review letters·2021

Search research articles

Related Experiment Video

Updated: Jul 11, 2026

Isolation of Human Islets from Partially Pancreatectomized Patients

Isolation of Human Islets from Partially Pancreatectomized Patients

Published on: July 30, 2011

Islet isolation under cGMP conditions: replacing the coil.

M R Garfinkel¹, M Connors, D Ostrega

¹Department of Surgery, The University of Chicago, Chicago, Illinois 60637, USA. mgarfink@surgery.bsd.uchicago.edu

Transplantation Proceedings

|June 15, 2004

Summary

A disposable blood warming system effectively replaces metal coils for islet isolation, meeting flow and temperature control needs. This innovation supports current good manufacturing practices (cGMP) compliance for clinical islet transplantation.

More Related Videos

Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

Published on: November 5, 2016

Evaluation of the Impact of a New Cooling Cell Processor System on Islet Cell Isolation Facility

Evaluation of the Impact of a New Cooling Cell Processor System on Islet Cell Isolation Facility

Published on: August 11, 2023

Related Experiment Videos

Last Updated: Jul 11, 2026

Isolation of Human Islets from Partially Pancreatectomized Patients

Isolation of Human Islets from Partially Pancreatectomized Patients

Published on: July 30, 2011

Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

Published on: November 5, 2016

Evaluation of the Impact of a New Cooling Cell Processor System on Islet Cell Isolation Facility

Evaluation of the Impact of a New Cooling Cell Processor System on Islet Cell Isolation Facility

Published on: August 11, 2023

Area of Science:

Biomedical Engineering
Cellular Therapy
Transplantation Science

Background:

Clinical islet transplantation is increasingly regulated under current good manufacturing practices (cGMP).
cGMP mandates validation of equipment cleaning and sterilization.
Current automated islet isolation uses a metal coil requiring complex cleaning and sterilization.

Purpose of the Study:

To assess a disposable blood warming system as a cGMP-compliant alternative to metal coils.
To evaluate the system's flow rate accommodation during pancreatic digestion.
To determine the system's heat exchange efficiency and temperature control capabilities.

Main Methods:

A pancreas digestion circuit was established using either a metal coil or a disposable Warmflo blood warming bag.
Temperature within the digestion chamber was monitored using a thermocouple.
Time to reach 37°C and temperature maintenance were recorded at various set temperatures.

Main Results:

The disposable blood warming bag accommodated flow rates of 150 and 300 mL/min.
The bag achieved 37°C in shorter or equivalent time compared to the metal coil.
Temperature maintenance was equivalent, with successful application in four human islet isolations.

Conclusions:

A disposable blood warming system offers adequate flow, heat exchange, and temperature control for islet isolation.
This system may simplify compliance with current good manufacturing practices (cGMP).
Facilitates the advancement of islet isolation processes toward regulatory standards.