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Related Experiment Videos

Improved in vitro function of islets using small intestinal submucosa.

E J Woods1, C M Walsh, R A Sidner

  • 1General BioTechnology, LLC, Harlan Biotechnology Center, Indianapolis, Indiana 46202, USA. Erik@gnrlbiotech.com

Transplantation Proceedings
|June 15, 2004
PubMed
Summary

Small intestinal submucosa (SIS) enhances human islet function during in vitro culture, improving viability for diabetes mellitus transplantation. This technique supports better islet morphology and insulin secretion compared to standard culture methods.

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MESENCHYMAL STEM CELLS.

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Endocrinology

Background:

  • Pancreatic islet transplantation is a promising alternative to insulin therapy for diabetes mellitus.
  • Current protocols require extended culture periods, potentially reducing islet viability and function.
  • Optimizing in vitro islet culture is crucial for successful transplantation outcomes.

Purpose of the Study:

  • To investigate the efficacy of porcine-derived small intestinal submucosa (SIS) in maintaining human islet function during prolonged in vitro culture.
  • To compare the functional and morphological integrity of human islets cultured on SIS versus standard culture inserts.
  • To determine if SIS improves islet survival and insulin secretory capacity.

Main Methods:

  • Human pancreatic islets were cultured for 3 weeks in CMRL 1066 medium with supplements.

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  • Islets were cultured on non-coated inserts or inserts coated with SIS (50-200 islets/condition).
  • Islet function was assessed weekly via glucose-stimulated insulin secretion (GSIS) and Dithizone staining for viability.

    • Main Results:

    • SIS-cultured islets demonstrated significantly higher stimulation index (SI) from week 2 onwards (P < .05).
    • Islets cultured on SIS maintained excellent morphology (>90% Dithizone positive) throughout the 3-week culture period.
    • Islets cultured on non-coated inserts showed a 'loose' morphology and reduced viability over time.

    Conclusions:

    • Porcine small intestinal submucosa (SIS) significantly enhances human islet function and viability during in vitro culture.
    • SIS provides a supportive microenvironment that preserves islet morphology and insulin secretory capacity.
    • SIS represents a promising biomaterial for optimizing islet culture protocols to improve transplantation success in diabetes mellitus.