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Updated: Aug 3, 2026

Examination of Thymic Positive and Negative Selection by Flow Cytometry
Published on: October 8, 2012
Critical functions for c-Myb at three checkpoints during thymocyte development
Timothy P Bender1, Christopher S Kremer, Manfred Kraus
1The Department of Microbiology, PO Box 800734, University of Virginia Health System, Charlottesville, Virginia 22908-0734, USA. tpb3e@virginia.edu
Abstract:
The transcription factor c-Myb is expressed throughout T cell development in the thymus. However, little is understood about c-Myb function because of the embryonic lethality of traditional Myb-null mutations. Using tissue-specific deletion to abrogate c-Myb expression at distinct stages of T cell development, we identify three points at which c-Myb activity is required for normal T cell differentiation: transition through the double-negative 3 stage, survival of preselection CD4(+)CD8(+) thymocytes, and differentiation of CD4 thymocytes. Thus, c-Myb is essential at several stages during T cell development in the thymus.
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